diff --git a/openvidu-server/src/main/java/io/openvidu/server/config/Dotenv.java b/openvidu-server/src/main/java/io/openvidu/server/config/Dotenv.java
index b5ff822a..b150b0a7 100644
--- a/openvidu-server/src/main/java/io/openvidu/server/config/Dotenv.java
+++ b/openvidu-server/src/main/java/io/openvidu/server/config/Dotenv.java
@@ -44,6 +44,10 @@ public class Dotenv {
 		return properties.get(property);
 	}
 
+	public Map<String, String> getAll() {
+		return properties;
+	}
+
 	public Path getEnvFile() {
 		return envFile;
 	}
@@ -55,26 +59,25 @@ public class Dotenv {
 	public void write(Path envFile) throws IOException {
 
 		List<String> outLines = new ArrayList<>();
-		
+
+		int lineNumber = 1;
 		for (String line : lines) {
-
 			try {
-
-				Pair<String, String> propValue = parseLine(envFile, line);
-				if(propValue == null) {
+				Pair<String, String> propValue = parseLine(envFile, line, lineNumber);
+				if (propValue == null) {
 					outLines.add(line);
 				} else {
-					outLines.add(propValue.getKey()+"="+properties.get(propValue.getKey()));
+					outLines.add(propValue.getKey() + "=" + properties.get(propValue.getKey()));
 				}
-
 			} catch (DotenvFormatException e) {
 				log.error("Previously parsed line is producing a parser error", e);
 			}
+			lineNumber++;
 		}
-		
-		if(!additionalProperties.isEmpty()) {
-			for(String prop : additionalProperties) {
-				outLines.add(prop+"="+properties.get(prop));
+
+		if (!additionalProperties.isEmpty()) {
+			for (String prop : additionalProperties) {
+				outLines.add(prop + "=" + properties.get(prop));
 			}
 		}
 
@@ -88,12 +91,13 @@ public class Dotenv {
 
 		lines = Files.readAllLines(envFile);
 
+		int lineNumber = 1;
 		properties = new HashMap<>();
 		for (String line : lines) {
 
 			log.debug("Reading line '{}'", line);
 
-			Pair<String, String> propValue = parseLine(envFile, line);
+			Pair<String, String> propValue = parseLine(envFile, line, lineNumber);
 
 			if (propValue != null) {
 
@@ -101,10 +105,12 @@ public class Dotenv {
 
 				properties.put(propValue.getKey(), propValue.getValue());
 			}
+
+			lineNumber++;
 		}
 	}
 
-	private Pair<String, String> parseLine(Path envFile, String line) throws DotenvFormatException {
+	private Pair<String, String> parseLine(Path envFile, String line, int lineNumber) throws DotenvFormatException {
 
 		if (isWhitespace(line) || isComment(line)) {
 			return null;
@@ -113,13 +119,15 @@ public class Dotenv {
 		int index = line.indexOf("=");
 
 		if (index == -1) {
-			throw new DotenvFormatException("File " + envFile + " has a malformed line with content \"" + line + "\"");
+			throw new DotenvFormatException("File " + envFile + " has a malformed line at position " + lineNumber
+					+ " with content \"" + line + "\"");
 		}
 
 		String property = line.substring(0, index).trim();
 
 		if (property.equals("")) {
-			throw new DotenvFormatException("File " + envFile + " has a malformed line with content \"" + line + "\"");
+			throw new DotenvFormatException("File " + envFile + " has a malformed line at position " + lineNumber
+					+ " with content \"" + line + "\"");
 		}
 
 		String value = line.substring(index + 1);
diff --git a/openvidu-server/src/main/java/io/openvidu/server/config/OpenviduConfig.java b/openvidu-server/src/main/java/io/openvidu/server/config/OpenviduConfig.java
index ee90d7bf..92cd0f6d 100644
--- a/openvidu-server/src/main/java/io/openvidu/server/config/OpenviduConfig.java
+++ b/openvidu-server/src/main/java/io/openvidu/server/config/OpenviduConfig.java
@@ -18,6 +18,7 @@
 package io.openvidu.server.config;
 
 import java.io.File;
+import java.io.IOException;
 import java.net.Inet4Address;
 import java.net.Inet6Address;
 import java.net.InetAddress;
@@ -52,6 +53,7 @@ import com.google.gson.JsonSyntaxException;
 import io.openvidu.java.client.OpenViduRole;
 import io.openvidu.server.OpenViduServer;
 import io.openvidu.server.cdr.CDREventName;
+import io.openvidu.server.config.Dotenv.DotenvFormatException;
 import io.openvidu.server.recording.RecordingNotification;
 
 @Component
@@ -421,29 +423,35 @@ public class OpenviduConfig {
 		this.configErrors.add(new Error(property, value, msg));
 	}
 
-	@PostConstruct
-	public void checkConfiguration() {
-
+	public void checkConfiguration(boolean loadDotenv) {
 		try {
-			this.checkConfigurationProperties();
+			this.checkConfigurationProperties(loadDotenv);
 		} catch (Exception e) {
 			log.error("Exception checking configuration", e);
 			addError(null, "Exception checking configuration." + e.getClass().getName() + ":" + e.getMessage());
 		}
-
 		userConfigProps = new ArrayList<>(configProps.keySet());
-
 		userConfigProps.removeAll(getNonUserProperties());
 	}
 
+	@PostConstruct
+	public void checkConfiguration() {
+		this.checkConfiguration(true);
+	}
+
 	protected List<String> getNonUserProperties() {
-		return Arrays.asList("server.port", "COTURN_IP", "COTURN_REDIS_IP", "KMS_URIS", "COTURN_REDIS_DBNAME",
-				"COTURN_REDIS_PASSWORD", "COTURN_REDIS_CONNECT_TIMEOUT");
+		return Arrays.asList("server.port", "DOTENV_PATH", "COTURN_IP", "COTURN_REDIS_IP", "KMS_URIS",
+				"COTURN_REDIS_DBNAME", "COTURN_REDIS_PASSWORD", "COTURN_REDIS_CONNECT_TIMEOUT");
 	}
 
 	// Properties
 
-	protected void checkConfigurationProperties() {
+	protected void checkConfigurationProperties(boolean loadDotenv) {
+
+		if (loadDotenv) {
+			dotenvPath = getValue("DOTENV_PATH");
+			this.populatePropertySourceFromDotenv();
+		}
 
 		serverPort = getValue("server.port");
 
@@ -489,7 +497,6 @@ public class OpenviduConfig {
 
 		checkCertificateType();
 
-		dotenvPath = getValue("DOTENV_PATH");
 	}
 
 	private void checkCertificateType() {
@@ -860,4 +867,51 @@ public class OpenviduConfig {
 		}
 	}
 
+	protected void populatePropertySourceFromDotenv() {
+		File dotenvFile = this.getDotenvFile();
+		if (dotenvFile != null) {
+			if (dotenvFile.canRead()) {
+				Dotenv dotenv = new Dotenv();
+				try {
+					dotenv.read(dotenvFile.toPath());
+					this.propertiesSource = dotenv.getAll();
+					log.info("Configuration properties read from file {}", dotenvFile.getAbsolutePath());
+				} catch (IOException | DotenvFormatException e) {
+					log.error("Error reading properties from .env file: {}", e.getMessage());
+					addError(null, e.getMessage());
+				}
+			} else {
+				log.error("OpenVidu does not have read permissions over .env file at {}", this.getDotenvPath());
+			}
+		}
+	}
+
+	public File getDotenvFile() {
+		if (this.getDotenvPath() != null && !this.getDotenvPath().isEmpty()) {
+			File file = new File(this.getDotenvPath());
+			if (file.exists()) {
+				if (file.isDirectory()) {
+					file = new File(file, ".env");
+					if (file.exists() && file.isFile()) {
+						return file;
+					} else {
+						log.error(".env file not found at folder {}", this.getDotenvPath());
+					}
+				} else {
+					if (".env".equals(file.getName())) {
+						return file;
+					} else {
+						log.error("DOTEN_PATH configuration property refers to a file which is not .env ({})",
+								this.getDotenvPath());
+					}
+				}
+			} else {
+				log.error(".env file not found at {}", this.getDotenvPath());
+			}
+		} else {
+			log.warn("DOTENV_PATH configuration property is not defined");
+		}
+		return null;
+	}
+
 }
\ No newline at end of file