diff --git a/openvidu-server/deployments/enterprise/master-node/docker-compose.yml b/openvidu-server/deployments/enterprise/master-node/docker-compose.yml
index edee3011..4fff5559 100644
--- a/openvidu-server/deployments/enterprise/master-node/docker-compose.yml
+++ b/openvidu-server/deployments/enterprise/master-node/docker-compose.yml
@@ -65,8 +65,6 @@ services:
         volumes:
             - ./:/opt/openvidu
             - /var/run/docker.sock:/var/run/docker.sock
-        env_file:
-            - .env
         environment:
             - SERVER_PORT=4443
             - SERVER_SSL_ENABLED=false